ACUTE TOXICITY AND EFFECT OF METHALONIC EXTRACT OF MORINGA OLEIFERA YOUNG PODS ON BLOOD GLUCOSE LEVEL IN NON DIABETIC RATS
*Ojo, N. A1.,Usman, A.A1.,Sambo, N2., Madziga, H. A1.,Yahi, D1., Mbaya, Y.
P1.,Balogun, S. T3. and Nnennaya, C. C.4
1Department of Veterinary Physiology, Pharmacology and Biochemistry
Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria
2 Department of Human Physiology, College of Medical Sciences, University of Maiduguri,
Borno State, Nigeria
3 Department of Clinical Pharmacology and Therapeutics, College of Medical Sciences,
University of Maiduguri,Borno State, Nigeria
4. Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Port
Harcourt. River State, Nigeria
Methalonic extract of young pods of Moringaoleifera was investigated for acute toxicity and effect on blood glucose level in non diabetic rats. The aqueous extract was subjected to phytochemical and elemental analysis. The phytochemical screening revealed the presence of tannins, flavonoids, carbohydrate, alkaloids, saponins, terpenoid,anthraquinone and cardiac glycoside.The elemental analysis in part per million [PPM] concentration showed 5.00 for calcium,0.41 for potassium,0.34 for copper,0.31 for iron,0.26 for fluorine,0.19 for nickel,0.16 for zinc, 0.12 for manganese and 0.03 for chromium .The acute toxicity studies using Lorke’s method showed that the extract did not produce death in treated rats at 5000mg/kg thus indicating that the extract was well tolerated. Administration of the extract to the rats for 21days at 200, 400 and 800 mg/kg body weight, produced significant (P<0.05) decreases in the blood glucose levels of the treated rats. The significant hypoglycemic effect may be the justification for Moringaoleiferafolkoloric use as a potential hypoglycemic agent. In conclusion, Moringaoleiferayoung pods showed a significant hypoglycemic effect with very wide safety margin.
Key words: Acute toxicity, methanolicextract, Moringaoleifera, young pods, glucose level,rats