COMPARATIVE STUDIES ON THE EFFECT OF TRYPANOSOMA BRUCEI AND DFMO (DIFLUORO METHYL ORNITHINE) ON HAEMATOLOGICAL PARAMETERS AND BODY WEIGHTS IN WISTAR STRAIN ALBINO RATS
Abdulhamid Baba Njobdi1, Mohammed Shaibu Auwal2, Nicholas Adetayo Ojo2, Muhammad Mustapha3, Jibrin Suleiman Bargu4, Abdullahi Shuaibu5 and Ismail Alhaji Mairiga3 1Department of Animal Health, College of Agriculture Jalingo, Taraba State, Nigeria.
2Department of Veterinary Physiology, Pharmacology and Biochemistry. Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
3Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, Borno State, Nigeria.
4Department of Human Anatomy, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria.
5Department of Animal Health and Production, Mohamet Lawan College of Agriculture, Maiduguri, Borno State, Nigeria.
The aim and objectives of this research is to study the effects of T. brucei and Difluoromethyl ornithine (DFMO) on parked cell volume (PCV), haemoglobin concentration (Hb), red blood cells (RBC) count and body weights in wistar strain albino rats. Twenty healthy wistar albino rats were used and divided into four experimental groups (A, B, C, and D) of five rats each. Feed and water were provided ad libitum to the experimental rats. Rats in Group A were used as a control. Rats in group B were intraperitoneally inoculated with 5.0 x 105/µL of T. brucei (Gboko strain, Benue State, Nigeria), while rats in group C were orally administered 2% (20 mg / ml) difluoro methyl ornithine (DFMO) W/V at the dose of (400 mg /kg) on daily basis for 21 days. The rats in group D were infected with 5 x 105/µL of T. Brucei and treated with oral doses of 2% (20 mg / ml) difluoro methyl ornithine (DFMO) at the dose of (400mg /kg) W/V on daily bases for 21 days. Packed cell volume (PCV), haemoglobin concentration (Hb), red blood cells (RBC) count and weights of the rats were determined at 3 days interval for 21 days post-infection. Decrease in PCV, RBC, Hb concentration and progressive weight loss was noticed in T. brucei infected, difluoro methyl ornithine (DFMO) treated and T. brucei infected and treated rats as from day 6 post-infection (Pi). Treatment of trypanosomosis with difluoro methyl ornithine (DFMO) is found to have deleterious effect on PCV, RBC, Hb concentration of wistar strain albino rats manifesting in progressive loss of body weight, culminating in mortality in some of the experimental rats. Application of difluoro methyl ornithine in the treatment of trypanosomosis in livestock should therefore be done with caution, particularly in the treatment of chronic cases. Conclusively, this research have shown that treatment of trypanosomosis with Difluoro methyl ornithine causes decrease in packed cell volume (PCV), haemoglobin concentration (Hb), red blood cells (RBC) count and body weight in wistar strain albino rats.
Keywords: Comparative studies, Body weights, DFMO, T. brucei, Albino rats